User: rahulraghavantv

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Posts by rahulraghavantv

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I want to know how data is normalized between control group and test group in differential rna seq analysis. Can somebody please explain or suggest some references
... I am a beginer in rnaseq data analysis. I want to know how data is normalized between control group and test group in differential rna seq analysis. In case of wet lab experiments we use house keeping genes to normalize the data . how does normalizing of data done in rna seq data analysis using tuxe ...
planet all alignment forum rna-seq written 3 months ago by rahulraghavantv0 • updated 3 months ago by Jennifer Hillman Jackson23k
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not able to use cufflinks output from galaxy in cummeRbund
... I am using online galaxy server for rnaseq differential analysis. I am not able to use cufflinkoutput from galaxy for cummerbund. the galaxy doesnot show option to select output as" .db file". I am getting cuffdiff output as ".sqlite" . and it is not compatible with cummerbund. Is there any s ...
cufflinks galaxy alignment forum rna-seq written 3 months ago by rahulraghavantv0
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regarding gff3 file for rna seq analysis (tuxedo workflow) in human
... I want to analyze the rna seq data using tuxedo work flow. i have two replicates for control and two replicates for test. But i dont know what is 'gff3' file for human transcripts. where do i get this gff3 file for whole human transcripts. ...
cufflinks tophat rna-seq written 4 months ago by rahulraghavantv0
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Comment: C: How to convert SRA Files to FASTAQ files in a 32 BIt windows computer ( beginner
... Thank you, From the link you gave me, I found that tool kit in galaxy but how do I install it ? I have decompressed the file. ...
written 2.8 years ago by rahulraghavantv0
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Comment: C: How to convert SRA Files to FASTAQ files in a 32 BIt windows computer ( beginner
... Thank you for your reply, I mentioned that my computer is 32 bit because I found that many of the versions of SRAtool kit works only in 64 bit. I did not see any options in galaxy for converting SRA to FASTAQ. ...
written 2.8 years ago by rahulraghavantv0
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Answer: A: How to convert SRA Files to FASTAQ files in a 32 BIt windows computer ( beginner
... Thank you very much for the reply. I have installed both SRAtoolkit and NGSFC . They are able to convert sra files to fastaq but those fastaq files are not giving me FASTA QC results in galaxy. Therefore, to confirm it I obtained a fastaq file from outside. And that fastaq file was perfectly opening ...
written 2.8 years ago by rahulraghavantv0
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How to convert SRA Files to FASTAQ files in a 32 BIt windows computer ( beginner here)
... How to convert SRA Files to FASTAQ files in a 32 BIt windows computer ( beginner here) ...
galaxy sra fastq written 2.8 years ago by rahulraghavantv0

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