User: Ismaelrymy

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Ismaelrymy0
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Posts by Ismaelrymy

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Reads with failing Kmer enrichment in FastQC - priming bias?
... Hi there! I am performing a quality check in a transcriptomic dataset before attempting a de-novo assembly. Duplication is high, as expected, but I did not expect this results in the k-mer graph: ![enter image description here][1] No sequence is overrepresented in FastQC, whereas this makes me th ...
quality kmer fastqc rna-seq written 9 weeks ago by Ismaelrymy0 • updated 9 weeks ago by Jennifer Hillman Jackson25k
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Normalization on Single End and Paired End reads
... Hi there. I want to assemble a transcriptome with Trinity using reads that are both paired- and single-end. Due to memory issues I have to normalize the reads, but the normalization process in Trinity is not able to handle this kind of data. Is there any application in Galaxy that I can use to norm ...
galaxy assembly written 3 months ago by Ismaelrymy0 • updated 3 months ago by Jennifer Hillman Jackson25k

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