User: aida.shahraki

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Posts by aida.shahraki

<prev • 4 results • page 1 of 1 • next >
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(Closed) Troubles in de novo assembly of an insect genome
... I need to assemble an insect genome. The genome size is around 500Mb. I have performed genome sequencing in illumina platform, 150bp paired-end reads. I have trimmed forward and reverse reads and each of this files is around 45Gb. I have tried to assemble this dataset using SOAPdenovo and Abyss, but ...
assembly soap abyss written 11 weeks ago by aida.shahraki10 • updated 11 weeks ago by Jennifer Hillman Jackson25k
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FTP upload problem -- Upload data size limit is set at 50 GB, per file
... I have a problem with uploading my data to usegalaxy.org. I was trying to make FTP link of my data (two files each about 90GB) and uploading them to the galaxy, but after uploading of about 100GB, I received "file already exists" error and the transfer stopped. I would these data further in the gala ...
url usegalaxy.org file upload size written 12 weeks ago by aida.shahraki10 • updated 12 weeks ago by Jennifer Hillman Jackson25k
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Find a specific sequence in WGS data
... Hello everyone, I am trying to assemble the genome of an insect and in the next step, I am interested in finding the sequence of a specific protein which I have a part of it. Due to time shortage, I don't want to go through the gene annotation and instead I want to find it directly. Can anyone help ...
assembly written 12 weeks ago by aida.shahraki10
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genome de novo assembly
... Hello, I have a paired-end 150 bp genome sequencing data with a different end quality.According to the fastQC analysis, the 10 last nucleotides of one read should be trimmed, while for the other one 30 nucleotides need to be trimmed. Can I trim different numbers of nucleotides from each read and t ...
assembly insert size paired-end length written 3 months ago by aida.shahraki10 • updated 3 months ago by Jennifer Hillman Jackson25k

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