User: aangajala

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aangajala0
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Posts by aangajala

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Markduplicate single end reads miRNA seq data
... I am trying to analyze a RNA seq, single end SRA dataset. For this do i have to perform Markduplicates as it was mentioned in the tutorial bellow. When i do so, I am seeing 70-80% duplicates.In the example provided in the tutorial, it was less than 10% for paired end data.I am wondering if I should ...
rna-seq written 6 months ago by aangajala0 • updated 6 months ago by Jennifer Hillman Jackson25k
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Comment: C: miRNA seq data TRIMMOMATIC
... Thanks so much, when i tried using SRA NCBI upload.it worked.Thanks. ...
written 6 months ago by aangajala0
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miRNA seq data TRIMMOMATIC
... I am trying to analyze microRNA sequencing data for 8 datasets ( each dataset is a cell line). I am able to run FASTQC and Multi-QC. Single end data.Per sequence quality, 8/8 passed. Sequence duplication level 8/8 failed.Persequence GC content 8 failed. So When I tried to use TRIMMOMATIC, it is show ...
fastqsanger rna-seq ncbi sra fastq written 6 months ago by aangajala0 • updated 6 months ago by Jennifer Hillman Jackson25k

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