User: nd1091

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nd10910
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Posts by nd1091

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Answer: A: Trinity run ended with error
... Jen, Thank you very much for replying. As I have concatenated all my left and right files together in separate files that is why the size is too big. I can reduce number of dataset to make it work, just wondering what is the largest size of data I can use to run trinity!! Regards, Nihar ...
written 24 days ago by nd10910
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Trinity run ended with error
... Hello, My trinity run ended with an error saying "tool error An error occurred with this dataset: Remote job server indicated a problem running or monitoring this job." I have no idea what should be done regarding this matter. Anyone else with similar issue? Nihar ...
rna-seq trinity written 24 days ago by nd10910
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Large datasets and memory usage
... Hi, My data set (multiple, 17 pair ended) has total size of 87 GB. I want to run trinity denovo assembly. But as I have to prepocess the raw files number of files are increasing and I have almost used up all my space. My proprcoessing steps are, FASTQC>Trimmomatic>FASTQC>fastq Interlacer&g ...
quota purge account download delete written 29 days ago by nd10910 • updated 27 days ago by Jennifer Hillman Jackson24k
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Answer: A: Trinity run preprocessing
... Jen, Thank you very much. I have 4 experimental conditions with each have 2-3 replicates and my goal is to characterize differential expression and gene quantification. So I was wondering if I should group my data sets according to the experimental conditions and then run QA/QC?!?! I am just making ...
written 4 weeks ago by nd10910
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Trinity run preprocessing
... I am new to Trinity and galaxy instance (https://usegalaxy.org/). I have 17 samples pair ended. I was wondering if I have to use trimmomatic or fastq quality trimmer before I concatenate left-reads and right-reads, and then execute trinity. Thanks, Nihar ...
rna-seq trinity written 4 weeks ago by nd10910

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