User: sinha.puja

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sinha.puja0
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9 months ago
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Posts by sinha.puja

<prev • 6 results • page 1 of 1 • next >
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Problem in cuffdiff
... Hi everyone...after doing cuffdiff when viewing the data in tabular format of gene differential expression testing I am finding no fold change values, no test statistics, p and q values are 1 and there is no significant found in all over the table...so how this is possible...explain...or I think I h ...
galaxy written 9 months ago by sinha.puja0 • updated 9 months ago by Jennifer Hillman Jackson25k
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RNA -sequencing reads
... I am using galaxy for RNA-seq data analysis...can anyone tell me that read 1 (R1) corresponds to forward reads and read (R2) correspond to reverse reads or its vice versa..??? ...
rna-seq written 9 months ago by sinha.puja0 • updated 9 months ago by Jennifer Hillman Jackson25k
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Query in uploading data in stringtie
... When I am using stringtie in galaxy, the one option is **Reference annotation to use for guiding the assembly process** so how to upload gtf reference annotation in the column. However I have downloaded the human gtf file from ensemble and it also showed in my history in galaxy but the column in str ...
galaxy written 9 months ago by sinha.puja0 • updated 9 months ago by Jennifer Hillman Jackson25k
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Cufflinks in galaxy
... When I am using cufflinks in galaxy the option use reference annotation, when selecting use reference annotation as guide it shows no gtf data set available but in Tophat I have used hg19 as a reference genome...so how could I select this....And also after running tophat the files shows it still run ...
galaxy written 9 months ago by sinha.puja0 • updated 9 months ago by Jennifer Hillman Jackson25k
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running of the files after uploading
... Hi... I am unable to run the files after uploading. The uploading is done but on the left side one clock like sign exists and when I am clicking on that it shows the file is waiting to run.Please help and guide so that I can use tophat and also in tophat section the second option RNA-Seq FASTQ file ...
galaxy written 9 months ago by sinha.puja0 • updated 9 months ago by Jennifer Hillman Jackson25k
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Problem in uploading files
... I am unable to upload files on galaxy..upon uploading files from computer it shows the file exceeds the maximum size. And when uploading ftp files it seeks username and password. After entering user name and password it shows incorrect. So how to resolve the problem..? ...
galaxy written 9 months ago by sinha.puja0 • updated 9 months ago by Jennifer Hillman Jackson25k

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