User: thomas.enriquez

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Posts by thomas.enriquez

<prev • 3 results • page 1 of 1 • next >
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Answer: A: Impossible to use Htseq-count on BAM files from Tophat2
... Hello , Sorry for the time withtout answers. So i set up Htseq count with the term "transcript_id" and I convert my gff3 into gtf using gffread. Htseq has finished his job without any error report, but the output matrix contains only 0 : Geneid TopHat on data 69 data 16 and data 15: accepted_hit ...
written 11 months ago by thomas.enriquez0
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Comment: C: Impossible to use Htseq-count on BAM files from Tophat2
... Dear Julie, thank you for responding. Yes, I want to count according gene_id. I'm using galaxy, and in Htseq you can specify the ID attribute, and I specified gene_id. Here is a link to a txt file with the line 40 of the gff3 file: https://www.dropbox.com/s/3b3gih4iqlzds43/exemple%20gff3.txt?dl= ...
written 12 months ago by thomas.enriquez0
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Impossible to use Htseq-count on BAM files from Tophat2
... Hello, I'm currently facing troubles using galaxy. I want to compare differentially expressed genes between two treatment groups. I already map my reads on my reference genome (70% remaping) and now I'm trying to obtain the differential expression matrix using Htseq count. (For information, my da ...
software error rna-seq written 12 months ago by thomas.enriquez0

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