User: pattythepsuwan

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Posts by pattythepsuwan

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Answer: A: Tophat vs Bowtie2
... Hi Jennifer, I am trying to analyze the Ribo-seq data. I cannot find any program that analyze the ribo-seq. However, I begin by using the RNA-seq analysis and first thing I do is mapped the data into BAM file. Any modules on Galaxy that recommend to use with Ribo-seq analysis? Also I am very new t ...
written 4 months ago by pattythepsuwan0
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Tophat vs Bowtie2
... Hello, I took the fasq files and ran Tophat then Bowtie2. Is it okay to run tophat first then bowtie2 or in reverse order. Please advice. Best, Patty Thepsuwan ...
bam bowtie tophat rna-seq written 4 months ago by pattythepsuwan0
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How to remove duplicate sequence
... Our Ribo-seq libraries involved a PCR step (9 cycles of amplification) in order to get enough material to put on the sequencer. Because of this, we expect that many of the reads are actually exact duplicates of clones which are not real duplicates but arise as an artifact of PCR. Is there any optio ...
duplicate reads rna-seq written 4 months ago by pattythepsuwan0 • updated 4 months ago by Jennifer Hillman Jackson23k

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