User: mm4184

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mm418420
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Posts by mm4184

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Getting all unmapped reads
... I have a fastqsanger dataset from an RNAseq experiment, and I am trying to remove contaminating human sequences. I can run BWA for Illumina against the human genome and get BAM files of the mapping, but I am trying to get a fastqsanger file with only the unmapped reads. I have been able get this as ...
bwa rna-seq written 15 months ago by mm418420 • updated 15 months ago by Guy Reeves1.0k
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splitting and rejoining datasets
... I am working with large fastq files which are too big to use on galaxy (about 120 gb, so even after purging everything else I still don't have enough space to do a quality trim on the data). Does anyone know if there are programs to split my dataset into pieces, which I could recombine after batch q ...
chip-seq written 15 months ago by mm418420 • updated 15 months ago by Jennifer Hillman Jackson25k

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