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Posts by vit.filippo
... Thank you Ms Hillman for your precious help. I've just tried hisat but I've had the very same problem. The total number of reads is 20*10^6 but the aligner is able to read only half of them. There are no errors suggesting that everithing is fine. Since the problem is not the quality, I've thought th ...
... Since I am using a laptop, I'd like to exploit usegalaxy to perform an alignment using tophat. I succeeded in doing the alignment, but bowtie2 read half of the reads. The reads were generated by an Illumina Hiseq 2000, the protocol wasn't strand specific and it was a single end experiment. - I per ...
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