User: xxtomnyxx

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xxtomnyxx0
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Posts by xxtomnyxx

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Comment: C: featureCounts gives extreme low counts on highly expressed genes
... Hum..... I think I know what's wrong.... I should set the "Allow read to contribute to multiple features" parameter in "Advanced options" to "Yes." So I guess the problem is caused by that the reads belong to GAPDH and T (and other genes with same problem) were already counted on other RefSeq featur ...
written 4 months ago by xxtomnyxx0
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featureCounts gives extreme low counts on highly expressed genes
... I mapped paired-end sequencing with RNA-STAR and got the BAM file. I then use featureCounts to count the reads with a gene annotation file (an GTF file generated from UCSC table browser). But I found that some genes have extreme low counts. I checked the BAM file with IGV and the BAM file shows lots ...
featurecounts rna-seq written 4 months ago by xxtomnyxx0

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