User: olivier.rousseau.1

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Posts by olivier.rousseau.1

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Work with protein sequence in snpeff?
... Hi, I am knew to bioinformatics and I would like to know if it is possible to run the snpeff on protein sequence? I have successfully identified the variant by nucleotide position, but i want to know the codon for each of my sequences. My goal is to identify residue mutation at specific positions. ...
protein snpeff ngs snpsift sequence written 15 months ago by olivier.rousseau.10 • updated 15 months ago by Jennifer Hillman Jackson24k
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Comment: C: NGS: Variant identification at specific positions in an enzyme engineering appro
... Hi, Thank you for your answer. The output gives me the variation for each nucleotide. Would it be possible to extract the information of mutation by residues. To look at the codon and the output would give me, for example, that the Leu75 was mutated to Lys75? and also the frequency of the residue m ...
written 15 months ago by olivier.rousseau.10
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Answer: A: NGS: Variant identification at specific positions in an enzyme engineering appro
... Hi, Thank you for your answer. The output gives me the variation for each nucleotide. Would it be possible to extract the information of mutation by residues. To look at the codon and the output would give me, for example, that the Leu75 was mutated to Lys75? and also the frequency of the residue m ...
written 15 months ago by olivier.rousseau.10
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NGS: Variant identification at specific positions in an enzyme engineering approach
... Hi, I am wondering how to extract the data of my NGS analysis. In my experience, I did site-directed saturation mutagenesis at 49 positions in my enzyme. So theoratically, i have all the possible mutations at each chosen positions. Then I did my own PCR fragment of 250 bp to avoid random sequences ...

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