User: Zhiqiang Shu

gravatar for Zhiqiang Shu
Zhiqiang Shu80
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Last seen:
5 years, 7 months ago
Joined:
5 years, 8 months ago
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z***@bio.fsu.edu

Posts by Zhiqiang Shu

<prev • 7 results • page 1 of 1 • next >
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Order Of Steps
... Hi, I have two simple questions related to manipulating step history. So, how to change the order of steps, for example, put step 7 right after step 3. Second question is related, how to re-order steps, for instance, I deleted step 4 and 5 and want the next step (step 6) to take the order of 4, jus ...
written 5.6 years ago by Zhiqiang Shu80 • updated 5.6 years ago by Jennifer Hillman Jackson25k
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Trim Adapters And Other
... Hi, all! I have a maybe naive question that might be not so related to Galaxy usage. So I got the RNA seq data from Illumina Hiseq. Do I need to get rid of the adapter manually? If so, is it "clip adapter" that I should use? It should only have one adapter sequence or maybe more than one? And the a ...
galaxy written 5.6 years ago by Zhiqiang Shu80
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Comment: C: Combining Two Fastq Files
... This is my question, too! In my case, I just want to combine two fastq files into only one, for instance, one file come first and then comes the second. How can I make it? Thanks! Zhiqiang Quoting "Peter Cock" : Zhiqiang Shu/Deng Lab Department of Biological Science Florida State University 3 ...
written 5.6 years ago by Zhiqiang Shu80
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Comment: C: Find Out Sense And Antisense Sequences
... Thank you so much, Jennifer! Your method is pretty good for filtering the sequences that have "A" at the 10th base position.But I'm sure if it have employed 10 bp complementary criterion? For the antisense, the 5' will be T (in complementary to A). Besides, my library contains other small RNAs. Tha ...
written 5.6 years ago by Zhiqiang Shu80
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Comment: C: Find Out Sense And Antisense Sequences
... Thank you, Peter! I think I should put more detailed information here. What I'm doing is piRNA data. Two groups of piRNA (named sense and antisense)are in the library. As I said, they are complementary to each other for about 10 nt, while the whole length is about 30nt. For the sense group, they sh ...
written 5.6 years ago by Zhiqiang Shu80
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Find Out Sense And Antisense Sequences
... Hi, Galaxy users! I have a question on how to find out sense and antisense sequence. I've got RNA seq data in the fastq format. The sequences inside are partially complementary to each other (complementary is 10nt, while entire is about 30nt). How can I separate these sequences into two groups: sen ...
galaxy written 5.6 years ago by Zhiqiang Shu80 • updated 5.6 years ago by Jennifer Hillman Jackson25k
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How To Show Sequence Length Data
... Hi, there I'm new to this field and have a question when using Galaxy. I have RNA sequence data. How can I plot the graph of the length of sequence with Galaxy. Thanks! ...
galaxy written 5.7 years ago by Zhiqiang Shu80 • updated 5.7 years ago by Peter Cock1.4k

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