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• Need help with "FASTQ Trimmer" tool
  FASTQ Trimmer seems unable to recognize my FASTQ files as input. For e.g. I carried out FASTQ to ...
• Need help with "FASTQ Quality Trimmer" tool
  I am not able to add my file to this tool.     Tool name: FASTQ Quality Trimmer Tool version:...
• Need help with "Barcode Splitter" tool
  i uploaded a fastq file of the output from the sequencing but it is not recognized as a "library"...
• Need help with "FASTQ Quality Trimmer" tool
  I have uploaded .fastq files successfully but the "FASTQ file" drop-down list doesn't show my .fa...
• Need help with "FASTQ Quality Trimmer" tool
  I have uploaded my fastQ file but it is not showing in drop down list of fastQ-Quality Trimmer to...
• Need help with "FASTQ to FASTA" tool
  Excuse me, How can I submit my local FASTQ files to convert with this tool?   Tool name: FAST...
• Need help with "Filter by quality" tool
  Why doesn't this tool recognize my fastq files? It won't let me run the tool on the fastq files I...
• Need help with "FASTQ joiner" tool
  I can't get my fastq that are located in my history   Tool name: FASTQ joiner Tool version: 1....
• Need help with "FASTQ Summary Statistics" tool
  I imported some NGS files from EBI, but they came in the FASTQ.gz format. Is there a way to extra...
• Need help with "Reverse-Complement" tool
  I have a fastq file uploaded and would like to get the reverse-complement. But it was not recogni...
• Need help with "FASTQ splitter" tool
  I was having difficulty with the FASTQ splitter, it was not finding my fastq file (an SRA-based F...
• Need help with "FASTQ Quality Trimmer" tool
  When using this tool it fails to recognize my data files for input. They are uploaded as fastq bu...
• Need help with "Filter SAM" tool
      Tool name: Filter SAM Tool version: 1.0.0 Tool ID: toolshed.g2.bx.psu.edu/repos/devteam/sa...
• Need help with "Filter by quality" tool
  which formet this tool gets?   Tool name: Filter by quality Tool version: 1.0.0 Tool ID: tools...
• Need help with "FASTQ Groomer" tool
  Hi, I try to groom a sequence from NCBI sequenced from ilumina Hiseq 2000 by BGI, it's running...
• Need help with "FASTQ Groomer" tool
  Hi, How do I upload if I have 2 files for paired-end sequence?   Thanks, Tina   Tool name:...
• Need help with "Barcode Splitter" tool
  Hi, I would like to use the new "enhanced" barcode splitter that was developed this past year. I...
• Barcode limit in "Barcode Splitter" tool
  Hello,  I was wondering what is the barcode limit to be run in the "Barcode splitter" tool. I h...
• Need help with "FASTQ Groomer" tool
      Tool name: FASTQ Groomer Tool version: 1.0.4 Tool ID: toolshed.g2.bx.psu.edu/repos/devteam...
• Need help with "SAM to FASTQ" tool
  Hello, To convert to FASTQ format, I saved a SAM formatted file in the History, however, this fi...
• Need help with "Principal Component Analysis" tool
  I want to convert DNA sequence into RNA sequence how to upload target DNA sequence. it is saying...
• Need help with "Principal Component Analysis" tool
      Tool name: Principal Component Analysis Tool version: 1.0.2 Tool ID: toolshed.g2.bx.psu.ed...
• Need help with "Tophat2" tool
  I have uploaded fastq file to the galaxy, I can see it on the right "history", but I can not see ...
• Need help with "Tophat for Illumina" tool
  I do not know why: "RNA-Seq FASTQ file:" can not been selected?      Tool name: Tophat for Il...
• Need help with "Filter sequences by length" tool
  How do I upload my fasta file? No options appear when I click the down arrow? Thanks! Jackie ...