I'm sorry for my naive question (but this is my first analysis)...
Analysis: 19 PCR targetting exons (1 protein)
Fastq aligned by BWA (with genome reference hg19) (output SAM) > SAMtools (SAM-to-BAM) (output BAM) > Mpileup SNP and indel caller (output bcf) then
BCFtools, want to convert BCF format to VCF but output is tabular. Why?
I want visualization and exploring using IGV. How?
Thank you for your help