Question: Issues On Rnaseq Since The Changeover
0
Elwood Linney • 180 wrote:
My histories seem to be stopping their processing around the
Tophat-cuffmerge steps since the change over of Galaxy online.
Sometimes a
red box appears over my history name but disappears in a minute or
less.
I am wondering, given the way the Toolshed now looks different for
RNAseq
work, if some changes have been made that are disallowing the
procedures I
used previously.
For example:
I run larger Fastq files (100 million to 200 million 50bp single-ended
reads per sample). I have been grooming my illumina fastq's, using
Tophat
(not Tophat2) for alignment, then Cuffmerge, then Cuffdiff. This
worked in
the past but my history are just getting thru Tophat or Cuffmerge and
stalling. Should I be doing this in a different manner?
This has been my second time around with these datasets after it was
suggested that I delete and start all over again.
Elwood Linney
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modified 5.1 years ago
by
Nate Coraor ♦ 3.2k
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written
5.1 years ago by
Elwood Linney • 180
