Question: Regarding Combining Several Txt Files And Producing One Fastq File
0
Jennifer Hillman Jackson ♦ 25k wrote:
Hi Yona,
To merge together multiple datasets this way, use the tool "Text
Manipulation -> Concatenate datasets tail-to-head". This works on two
datasets at a time, so you may need to run it a few times if you have
more than that, adding in a new file to the master merged file with
each
run.
Watch out for introducing blank lines (unintentionally) between the
files. To remove them should any be present (it doesn't harm a file if
none are there), after you have merged all the files together, use the
tool "Filter and Sort -> Select" with:
option: NOT Matching
and the expression: ^$
Once you are sure that the merged file is correct, you can permanently
delete the working files to recover disk space. "FastQC" and/or "FASTQ
Groomer" are generally both good at detecting format problems.
http://wiki.galaxyproject.org/Support#Error_from_tools
Good luck with your project,
Jen
Galaxy team
--
Jennifer Hillman-Jackson
Galaxy Support and Training
http://galaxyproject.org