Question: Raw Count Of Genes
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gravatar for Els Willems
5.1 years ago by
Els Willems30
Els Willems30 wrote:
Dear all, I am a Phd student working on chicken genomics, with limited experience in the bio-informatics field. I performed an RNA-Seq experiment with single end 50 bp reads to find differential gene expressions between different groups. I have mapped this data with Tophat. Does anyone know how I can get the raw data count out of galaxy in a tab-delimited file? I would like to perform a statistical analysis in other software, without the RPKM normalization that cufflinks offers. Thank you very much. Have a nice day. Yours Sincerely, Els Ir. Els Willems KU Leuven Department of Biosystems Division Livestock - Nutrition - Quality Laboratory of Livestock Physiology Kasteelpark Arenberg 30 bus 2456 B - 3001 Heverlee T (+32) 016 32 17 29 F (+32) 016 32 19 94
rna-seq cufflinks • 939 views
ADD COMMENTlink modified 5.1 years ago by Björn Grüning350 • written 5.1 years ago by Els Willems30
0
gravatar for Björn Grüning
5.1 years ago by
Björn Grüning350 wrote:
Hi Els, try the tool 'Group data by a column and perform aggregate operation on other columns.' And choose 'count' or 'count distinct. Hope that will work. Happy research! Bjoern
ADD COMMENTlink written 5.1 years ago by Björn Grüning350
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