Question: Tophat
0
gravatar for Jennifer Hillman Jackson
5.9 years ago by
United States
Jennifer Hillman Jackson25k wrote:
Hello, Using the defaults and then testing the resulting SAM output seems to be what most folks are doing if they do not have access to the original library construction methods (e.g. size selection). Both SAM Tools and Picard are in Galaxy. This is a useful post where the options are discussed: http://www.biostars.org/post/show/16556/estimate-insert-size-in- paired-endmate-pair/ Is the data Illumina? The data source may be able to tell you if the adapter sequence was actually sequenced and/or if it was removed already or not. If present or you just suspect it is present, they would also have access to the Illumina fasta adapter data. You could also test with FastQC (before or after alignment, maybe on just a sample), then perform a clip based on those results, and re-run. See the tools in 'NGS: QC and manipulation' to perform these tasks. Going forward, please send questions as a new thread directly to our mailing list at galaxy-user@bx.psu.edu. http://wiki.g2.bx.psu.edu/Support#Public_mailing_list_Q_.26_A_discussi ons Best, Jen Galaxy team -- Jennifer Jackson http://galaxyproject.org
galaxy • 528 views
ADD COMMENTlink written 5.9 years ago by Jennifer Hillman Jackson25k
Please log in to add an answer.

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 16.09
Traffic: 128 users visited in the last hour