Question: Re : % On-Off Target
0
Antoine ROUSSELIN • 20 wrote:
Hello,
My English is very bad, it's a shame!
My I have a file for my baits (create a bed file of your Agilent
SureSelect targets with exons and introns captured). Sequencing
output, I get the files. Bam, my result sets of alignments on the
human genome complete (with CASAVA). I try to get the coverage I only
exonic regions (+ or - 50 bp).
Thanks
ROUSSELIN Antoine
Clinical Biology and Oncology Laboratory
Centre François BACLESSE
France/Caen
a.rousselin@baclesse.fr
Tel : (33) 02.31.45.40.44
Fax : (33) 02.31.45.50.53
De: Ross [mailto:ross.lazarus@gmail.com]
Date: mer. 12/21/2011 03:16
Ŕ: Antoine ROUSSELIN
Cc: galaxy-user@lists.bx.psu.edu
Objet : Re: [galaxy-user] % On-Off target
Hello Antoine,
I'm not sure I really understand your question but if the metrics
described
at http://picard.sourceforge.net/picard-metric-
definitions.shtml#HsMetrics are
of use, you could try the picard hybrid selection metrics Galaxy tool
or
use it on the command line. Otherwise perhaps you can get a more
helpful
response if you provide a clear explanation of the data formats you
have
and the measures you want.
On Wed, Dec 21, 2011 at 2:59 AM, Antoine ROUSSELIN
--
Ross Lazarus MBBS MPH;
Associate Professor, Harvard Medical School;
Head, Medical Bioinformatics, BakerIDI; Tel: +61 385321444;
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modified 6.9 years ago
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fubar ♦ 1.1k
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6.9 years ago by
Antoine ROUSSELIN • 20
