Question: Gene Ontology Help
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gravatar for Nicole Lynn Jacobs
7.0 years ago by
Nicole Lynn Jacobs10 wrote:
To Whom it May Concern: My name is Nicole McDaniels. I am a Ph.D. candidate at Syracuse University. I am attempting to use RNAseq to look at changes in the transcriptome of zebrafish embryos upon morpholino injection. I have been using Galaxy Main to manage my data thus far. Recently, I completed the cufflinks/cuffcompare/cuffdiff analysis. I now have cuffdiff files in the tabular format. I would like to assess the gene ontologies of the differentially expressed transcripts, but I'm having trouble figuring out how to transfer over to GO Galaxy and then what exactly I should be doing in GO Galaxy. Is there a different program or server that would be better/easier for the gene ontology analysis than GO Galaxy? I've also looked at the GOseq R package, but again I'm not sure about the tabular file format or how to change it to a more appropriate format. Any help you can give me with this would be greatly appreciated. I thank you in advance for your time. All the best, Nicole McDaniels Ph.D Candidate Syracuse University Department of Biology
rna-seq cufflinks • 1.3k views
ADD COMMENTlink modified 3.5 years ago by ajmaninisha30 • written 7.0 years ago by Nicole Lynn Jacobs10
0
gravatar for ajmaninisha
3.5 years ago by
ajmaninisha30
Canada
ajmaninisha30 wrote:

hi,

 

were you able to figure out how to use cuffdiff output for GO . Kindly provide me your email 

 

 

 

Nisha

ADD COMMENTlink written 3.5 years ago by ajmaninisha30
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