Question: Cufflinks Transcript-Extract
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gravatar for dongdong zhaoweiming
7.1 years ago by
dongdong zhaoweiming110 wrote:
Hi,   I use cufflinks to assemble my RNA-Seq reads which produced a GTF file, such as follows:(1)chr1 Cufflinks transcript 6234876 6235400 1000 +chr1 Cufflinks exon 6234876 6235060 1000 +chr1 Cufflinks exon 6235239 6235400 1000 + (2)chr1 Cufflinks transcript 6230065 6230437 1000chr1 Cufflinks exon 6230065 6230437 1000 I want to extract transcrip sequence from the (1) and (2). But fetch sequence could only extractgenomic sequence, it only fit for transcrit which had one exon,so are there any other tools to extract transcripts which had >=2 exons sequence and combine it? Thanks a lot!weimin zhao
rna-seq cufflinks • 1.2k views
ADD COMMENTlink modified 7.1 years ago by vasu punj360 • written 7.1 years ago by dongdong zhaoweiming110
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gravatar for vasu punj
7.1 years ago by
vasu punj360
vasu punj360 wrote:
You should be able to do in Biomart I think fetch seq shoudl also allow you to do the same. Vasu ________________________________ Subject: Fwd: [galaxy-user] Cufflinks transcript-extract Hi, I use cufflinks to assemble my RNA-Seq reads which produced a GTF file, such as follows:(1)chr1 Cufflinks transcript 6234876 6235400 1000 + chr1 Cufflinks exon 6234876 6235060 1000 + chr1 Cufflinks exon 6235239 6235400 1000 +   (2) chr1 Cufflinks transcript 6230065 6230437 1000 chr1 Cufflinks exon 6230065 6230437 1000   I want to extract transcrip sequence from the (1) and (2). But fetch sequence could only extract genomic sequence, it only fit for transcrit which had one exon,so are there any other tools to extract transcripts which had   Thanks a lot! weimin zhao ___________________________________________________________The Galaxy User list should be used for the discussion ofGalaxy analysis and other features on the public serverat usegalaxy.org.  Please keep all replies on the list byusing "reply all" in your mail client.  For discussion oflocal Galaxy instances and the Galaxy source code, pleaseuse the Galaxy Development list: http://lists.bx.psu.edu/listinfo/galaxy-devTo manage your subscriptions to this and other Galaxy lists,please use the interface at: http://lists.bx.psu.edu/
ADD COMMENTlink written 7.1 years ago by vasu punj360
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