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Question: NGS Assembly tutorial sample data question
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gravatar for jimhu
9 weeks ago by
jimhu • 20
jimhu • 20 wrote:

This dataset is used for some of the tutorials on Galaxy Training

https://zenodo.org/record/582600#.W6PYBS-ZPUI

The mutantR1 and mutantR2 fastq files are from paired ends, but I can't find information about the fragment size. Anyone know? If not, I may just map the reads and try to figure it out.
assembly tutorial data • 135 views
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modified 9 weeks ago by Jennifer Hillman Jackson ♦ 25k • written 9 weeks ago by jimhu • 20
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gravatar for Jennifer Hillman Jackson
9 weeks ago by
Jennifer Hillman Jackson ♦ 25k
United States
Jennifer Hillman Jackson ♦ 25k wrote:

Hello,

Yes, map the data then use a tool such as NGS: DeepTools > bamPEFragmentSize Estimate the predominant cDNA fragment length from paired-end sequenced BAM files.

Thanks! Jen, Galaxy team
ADD COMMENT • link written 9 weeks ago by Jennifer Hillman Jackson ♦ 25k
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