Question: (Closed) How to use Deseq2 to merge the biological triplicates (for two seperate conditions) for analysing differential expression of genes
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Novice • 0 wrote:
I am very new to R, I have used Deseq2 package after my feature counts. I have 2 stages of control and treated with triplicates of each. I am forwarding the code that I have used
step 1 : dds <- DESeqDataSetFromMatrix(countData = Feature_counts$counts, colData = colData, design = ~ Condition+Replicates) step 2 temp_dds <- DESeq(dds) step 3
res<-results(temp_dds, contrast=c("Condition","Control","treated"))
Can you please tell me that how can I merge the biological replicates as I have three. Am I doing it correct
Please I would really appreciate your guidance on this
Hello Novice!
We believe that this post does not fit the main topic of this site.
For line command usage help with DeSeq2, please try this forum instead https://support.bioconductor.org/
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