Question: Quality Assessment of RNA de novo assemble (trinity) without reference genome
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sdbaney • 10 wrote:
Hello, Are there any tools within the galaxy tool shed that would give me a quality assessment of my trinity assembly without using a reference genome (as there is none for my organism)? I ran two assemblies with the same data trimmed with different parameters and I am trying to figure out a way to determine which one yielded a better assembly. Unfortunately, all of the quality assessment tools uses a reference genome to map the transcripts back to it which I don't have.