Question: Deseq2 with 0 lines in output
2
gravatar for ewalker
12 weeks ago by
ewalker30
ewalker30 wrote:

Hi,

Using Galaxy main usegalaxy.org) I have been happily runnning Salmon followed by Deseq2 to get normalized counts on some small RNAseq data. Up until Mon 21 May 07:37:49 PM(UTC) all ran just fine. But the next time I tried to use Deseq2 on a different Salmon set at 8:20:28, I got an empty file as a result (0 lines). Repeated rerun of Salmon and deseq2 have resulted in the same. I have searched through every parameter, and have not done anything differently, and have reached the end of my own troubleshooting capabilities.

Weirdly, if I rerun that Deseq2 job from Mon 21 May 07:37:49 PM(UTC), it returns the data just fine like it did the first time. It seems like there is something changed about how Salmon output happens now, since my 'old' Salmon outputs (from earlier yesterday) work in Deseq2 but my newer Salmon outputs won't work, and give 0 lines after running Deseq2.

Thanks for any help you can give.

Elsbeth

salmon deseq2 • 128 views
ADD COMMENTlink modified 12 weeks ago by Jennifer Hillman Jackson25k • written 12 weeks ago by ewalker30
0
gravatar for Jennifer Hillman Jackson
12 weeks ago by
United States
Jennifer Hillman Jackson25k wrote:

Hello,

This sounds very much like a bug. We are finalizing the 18.05 release and would like to make sure this is not a native Galaxy problem versus a tool bug.

Would you please send in a shared history link to galaxy-bugs@lists.galaxyproject.org from your registered account email address? It is important to make sure all datasets are left undeleted.

In the same email, describe the data/tool combinations (briefly) and attach to a dataset number -- some matrix like the one below. Just reference one dataset per output to keep it simple, even though there can be multiple produced by the tool per job. The rest we'll be able to trace back to the input datasets used, tool versions, etc to find out what is going on.

new input/old output (N)
new input/new output (N)
old input/old output (N)
old input/new output (N)
* where "N" is the output dataset number

How to report a bug: https://galaxyproject.org/issues/#usage-problem-reporting

Thanks for reporting the problem! Jen, Galaxy team

ADD COMMENTlink modified 12 weeks ago • written 12 weeks ago by Jennifer Hillman Jackson25k
1

Done. Thank you, Jen

ADD REPLYlink written 12 weeks ago by ewalker30
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