I am a complete newbie to RNA-seq analysis. I am trying to follow online tutorials etc to learn the analysis by myself. I only have a bog-standard office desktop PC to work on, so I'm trying to do everything (as far as possible) in Galaxy.
Trying to map reads with RNA STAR, it is asking for a 'gene model for splice junctions'. Based on the information I have found on other forums, I have downloaded the genome file and the annotation file, but the instructions after that invariably require running STAR on my local machine. Is it possible to generate this gene model within Galaxy itself? If not, are there other workarounds?
Any suggestions immensely appreciated!