Question: How do I get +/- strand orientation info from my RNAseq run?
0
gravatar for daniel.kim
11 days ago by
daniel.kim30
Qiagen Sciences - Frederick Maryland
daniel.kim30 wrote:

I made a RNAseq library using a stranded-RNAseq-library prep kit. The kit only amplifies the transcript strand. I then aligned the paired-end fastq's using HISAT2 using the option for "stranded" and FR (forward read). Typically I use featureCounts to count the genes but I now need strand orientation info in my featureCounts table.

My question is how do I get strand info?

Thanks!!!!

ADD COMMENTlink modified 10 days ago by Jennifer Hillman Jackson25k • written 11 days ago by daniel.kim30
1
gravatar for Jennifer Hillman Jackson
10 days ago by
United States
Jennifer Hillman Jackson25k wrote:

Hello,

Set these two parameters:

  • Options for pair-end reads, specify the orientation with the parameter Orientation of the two read from the same pair.

  • Advanced Options, specify that the input is stranded with the parameter Strand specificity of the protocol.

The tool form format has changed slightly since this Galaxy tutorial was created, but the methods/options described are still valid:

All tutorials: https://galaxyproject.org/learn/

Thanks! Jen, Galaxy team

ADD COMMENTlink written 10 days ago by Jennifer Hillman Jackson25k
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