Question: Which Galaxy tool can concatenate fastq files from different lanes of flow-cell?
0
gravatar for daniel.kim
6 months ago by
daniel.kim30
Qiagen Sciences - Frederick Maryland
daniel.kim30 wrote:

Which tool on Galaxy can I use to merge my fastq files from lanes 1,2,3, and 4 of a single library (paired end reads) of an Illumina NextSeq run? That way I don't have to do an alignment for each lane individually...

ADD COMMENTlink modified 6 months ago by Jennifer Hillman Jackson25k • written 6 months ago by daniel.kim30
2
gravatar for Jennifer Hillman Jackson
6 months ago by
United States
Jennifer Hillman Jackson25k wrote:

Hello,

Use the tool: Text Manipulation: Concatenate datasets tail-to-head

Do this once for the forward reads, then again for the reverse. Keeping the files in the same order with both runs will help.

Thanks! Jen, Galaxy team

ADD COMMENTlink modified 6 months ago • written 6 months ago by Jennifer Hillman Jackson25k

Thank you Jennifer!

As always your answer was prompt and on the money!

ADD REPLYlink written 6 months ago by daniel.kim30
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