Question: (Closed) Troubles in de novo assembly of an insect genome
8 months ago by
aida.shahraki • 10
aida.shahraki • 10 wrote:
I need to assemble an insect genome. The genome size is around 500Mb. I have performed genome sequencing in illumina platform, 150bp paired-end reads. I have trimmed forward and reverse reads and each of this files is around 45Gb. I have tried to assemble this dataset using SOAPdenovo and Abyss, but the process has killed for each try. Actually, I am running Abyss on a system with fairly good RAM capacity (128GB) but still, it is killed. I have tried at 64 and 96 Kmers. I do really need to assemble dataset as soon as it is possible, so I would be so grateful if anyone help me how can I tackle the problem.
ADD COMMENT • link •written 8 months ago by aida.shahraki • 10
We believe that this post does not fit the main topic of this site.
This forum is for questions about using Galaxy https://galaxyproject.github.io/. For general bioinformatics help, please try https://www.biostars.org.
For this reason we have closed your question. This allows us to keep the site focused on the topics that the community can help with.
If you disagree please tell us why in a reply below, we'll be happy to talk about it.