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• FASTQC Overrepresented Sequences
  Hey all, after running Trimmomatic and clipping Illumina adapters, I always run a FASTQC to have...
• Nextera pair end adapter cleaning
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• How do i remove multiple adapter sequences from my RNAseq reads?
  Hi there, I want to remove the universal adapters as well as the index adapters in each data fil...
• Query regarding clipping
  I want to use clip adapter sequences.I have uploaded ther file. But my file did not appear in the...
• Clip Adapter Sequence
  Good morning, I am very new in using Galaxy. I would like to use Clip to remove the adapter sequ...
• Trim Adapters And Other
  Hi, all! I have a maybe naive question that might be not so related to Galaxy usage. So I got th...
• clip adapter sequences
  Hello, I am trying to clip adapter sequences from fastq files, however when I open this tool it ...
• Cip adapters with FASTX Galaxy
  I am just trying my first NGS analysis using Galaxy. FASTQC showed contamination with adapter dim...
• Clip
  Hi, I was using the clip program, but my adapters are on the 5' end. Is there any way to use th...
• Trimming Adapters for Illumina Small RNA Libraries
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• adding illumina adapter sequences to local galaxy installation for use with trimmomatic
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• Paired end illumina RNAseq reads - running trinity with paired trimmed files and adding unpaired as single read?
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• Trim Galore version - Adapter sequence
  Hello, I have been using Galaxy's Trim galore for clipping adapters from Illumina small RNA sequ...
• Trimmomatic and adapters
  Hi all, As relatively new within the bioinformatics world, I am a bit confused when it comes to ...
• Removing a single base from 3' end of the reads using "Clip" option
  Hello, I need to remove a single base i.e. C from the 3' end of all of my reads. I tried doing i...
• trimmomatic with paired-end
  Dear Biostar, I have paired-end sequencing data I would like to trim using trimmomatic. I have r...
• how can I use a custom adapter sequence in trimmomatic while in Galaxy?
  Hi, I want to use a custom adapter sequences in trimmomatic. But when I upload a fasta file, the...
• Primer Contamination, Miranalyzer
  Hi Galaxy, Ive got 2 problems for you; 1) Ive got microRNA Illumina NGS data that I want to ana...
• Trimmomatic on paiera end data
  Hi I am trying to use Trimmomatic on my Sanger Illumina 1.9 paired end data. Trimmomatic ask fo...
• 3' Adapter Trimming Using Fastx-Toolkit Clipper
  Hi all, I am analyzing miRNA sequencing now. My data is 51bp, single -ended and ~5 M reads. I wan...
• What is the sequence of Illumina Universal Adapters?
  I am currently analyzing RNA-seq data. I would anyways be trimming the adapters. However, it woul...
• Trimmomatic works good?
  Hi, I am newbie in data analysis of RNAseq. I have tried to remove the illumna adapters by trimmo...