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Question: How to prepare FastQ files for HISAT2
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gravatar for skhan
9 months ago by
skhan • 40
skhan • 40 wrote:

Hi there,

Should I be preparing or otherwise cleaning fastq files before I try to align them to a reference genome using HISAT2? If yes, how and using which tools?

I have 2x 76 bases paired end (separate R1 and R2 files) reads and am attempting RNA-Seq.

Thanks.
fastq alignment rna-seq hisat2 • 657 views
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modified 8 months ago by Jennifer Hillman Jackson ♦ 25k • written 9 months ago by skhan • 40
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gravatar for Jennifer Hillman Jackson
8 months ago by
Jennifer Hillman Jackson ♦ 25k
United States
Jennifer Hillman Jackson ♦ 25k wrote:

Hello,

Please see the Galaxy tutorials here: https://galaxyproject.org/learn/

Start with these:

  • NGS logistics - this is an introduction to Galaxy's functionality for the analysis of Next Generation Sequencing data.
  • RNA-seq: Introduction - an introductory tutorial for transcriptome analysis.
  • RNA-seq: Discovering and quantifying new transcripts - an in-depth transcriptome analysis example.

Thanks! Jen, Galaxy team
ADD COMMENT • link written 8 months ago by Jennifer Hillman Jackson ♦ 25k
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