Question: Are reads number normalized on transcript length ?
0
gravatar for simomll
10 months ago by
simomll0
simomll0 wrote:

Hello,

I used featurecounts to obtain reads number from a RNA-seq file (.bam). I would know if the reads obtained are normalized on transcript length or not. Featurecounts doesn't specific that

Thank you for helping

rna-seq featurecounts • 332 views
ADD COMMENTlink modified 10 months ago by Jennifer Hillman Jackson25k • written 10 months ago by simomll0
0
gravatar for Jennifer Hillman Jackson
10 months ago by
United States
Jennifer Hillman Jackson25k wrote:

Hello,

Featurecounts does not normalize - these are straight counts. http://bioinf.wehi.edu.au/featureCounts/

Other RNA-seq tools can normalize. See the tool forms, manuals, and/or our tutorials for usage help.

Thanks! Jen, Galaxy team

ADD COMMENTlink written 10 months ago by Jennifer Hillman Jackson25k
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