Question: Are reads number normalized on transcript length ?
0
gravatar for simonemollame
3 months ago by
simonemollame0 wrote:

Hello,

I used featurecounts to obtain reads number from a RNA-seq file (.bam). I would know if the reads obtained are normalized on transcript length or not. Featurecounts doesn't specific that

Thank you for helping

rna-seq featurecounts • 107 views
ADD COMMENTlink modified 3 months ago by Jennifer Hillman Jackson24k • written 3 months ago by simonemollame0
0
gravatar for Jennifer Hillman Jackson
3 months ago by
United States
Jennifer Hillman Jackson24k wrote:

Hello,

Featurecounts does not normalize - these are straight counts. http://bioinf.wehi.edu.au/featureCounts/

Other RNA-seq tools can normalize. See the tool forms, manuals, and/or our tutorials for usage help.

Thanks! Jen, Galaxy team

ADD COMMENTlink written 3 months ago by Jennifer Hillman Jackson24k
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