Question: Are reads number normalized on transcript length ?
gravatar for simomll
7 months ago by
simomll0 wrote:


I used featurecounts to obtain reads number from a RNA-seq file (.bam). I would know if the reads obtained are normalized on transcript length or not. Featurecounts doesn't specific that

Thank you for helping

rna-seq featurecounts • 227 views
ADD COMMENTlink modified 7 months ago by Jennifer Hillman Jackson25k • written 7 months ago by simomll0
gravatar for Jennifer Hillman Jackson
7 months ago by
United States
Jennifer Hillman Jackson25k wrote:


Featurecounts does not normalize - these are straight counts.

Other RNA-seq tools can normalize. See the tool forms, manuals, and/or our tutorials for usage help.

Thanks! Jen, Galaxy team

ADD COMMENTlink written 7 months ago by Jennifer Hillman Jackson25k
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