11 months ago by
This error message comes out when there is a problem with the inputs. The current version of Diffbind does not have any known issues, so you do not need to consider a server-side problem if working at https://usegalaxy.org.
The important part of the error message can be seen after clicking on the bug icon (but you don't need to send this in since I've already looked at your history). This warning message can indicate that there is a mixup in the inputs.
No sites above threshold
Error in pv.getPlotData(DBA, attributes = attributes, contrast = contrast, :
Unable to plot -- no sites within threshold
Calls: dba.plotHeatmap -> pv.getPlotData
The given names of the inputs also seem to indicate that there might be a mixup between the BAM/BED pairs for some samples. But these are custom names, so you'll need to trace back through prior steps to find out which are the proper file pairs to use and/or if an earlier job/step didn't actually use the intended input data.
I didn't notice any obvious data content issues, but just for reference I'll give you the link to the support FAQs that help address those in case any are a factor once you dig into the problem: https://galaxyproject.org/support/#troubleshooting
Hope that helps! Jen, Galaxy team