Question: problem in transcriptome assembly
0
gravatar for majeedaasim
3 months ago by
majeedaasim0 wrote:

I successfully assembled my raw reads to generate assembly. But when I concatenate the reads, the assembly fails in Trinity . I used Trinity on galaxy as well but there too it failed. what could be the problem.

Thanks

assembly • 112 views
ADD COMMENTlink modified 3 months ago by Jennifer Hillman Jackson23k • written 3 months ago by majeedaasim0
0
gravatar for Jennifer Hillman Jackson
3 months ago by
United States
Jennifer Hillman Jackson23k wrote:

Hello,

The original reads should be entered individually in fastq format when using Trinity in Galaxy. Trinity command-line will also accept fasta format but individual reads are still the expected input.

Trinity manual: https://trinityrnaseq.github.io/

Galaxy tutorials: https://galaxyproject.org/learn/

Hope this helps, Jen, Galaxy team

ADD COMMENTlink written 3 months ago by Jennifer Hillman Jackson23k
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