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Question: blastx evalue problem
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gravatar for rahul.sg98
17 months ago by
rahul.sg98 • 0
rahul.sg98 • 0 wrote:

Hello, I am using galaxy blastx and giving cut off e-value of 1.0e-5, but still e-value more than 1 is appearing in the result.Any suggestions are welcome.

regards Rahul
blastx • 410 views
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modified 17 months ago by Jennifer Hillman Jackson ♦ 25k • written 17 months ago by rahul.sg98 • 0
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gravatar for Jennifer Hillman Jackson
17 months ago by
Jennifer Hillman Jackson ♦ 25k
United States
Jennifer Hillman Jackson ♦ 25k wrote:

Hello,

Reformat the value to be: 1e-05

Are you using Galaxy or running the tool command line or both? I see your other post at Biostars where the command-line was used: https://www.biostars.org/p/259182/

If you are having problems with the Galaxy version, please make sure that the most current version of Galaxy (15_07) is being used along with the most current version of the Blast+ tool wrapper (0.2.00).

Publication for the Blast+ wrapper in Galaxy: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4557756/

Thanks, Jen, Galaxy team
ADD COMMENT • link written 17 months ago by Jennifer Hillman Jackson ♦ 25k

Thanks for your comment. you are right, I am running Blastx on galaxy and command line, but on both cases, i am facing the problem. In case of Command line I have tried.. e-value cut off 0.00001, 1.e-5,1.0e-5,1.0e-05 ,but problem remains the same.

regards Rahul

ADD REPLY • link written 17 months ago by rahul.sg98 • 0
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