Latest
Open
RNA-Seq
ChIP-Seq
SNP
Assembly
Forum
Planet
All »
Home
Log In
Question: Reference genome: no options available - use Custom reference genome
0
gravatar for a.donato
17 months ago by
a.donato • 0
a.donato • 0 wrote:

Hi,

I am using galaxy and in particular a modified version of a workflow that I found on usegalaxy.org.

Even if in the workflow I have everywhere indicated which one is the reference genome I wish to use, some steps such as the "Realigner target creator" on data coming from the step "Add or replace groups" give me a error because in the using reference genome section there are no options available and I don't know why that's happening and how to fix it.

Can anyone with more experience than me explain me how I should proceed?

Thank you.

Best regards. Alessandra
tool reference genome fasta custom-genome • 469 views
ADD COMMENT • link •
modified 17 months ago by Jennifer Hillman Jackson ♦ 25k • written 17 months ago by a.donato • 0
0
gravatar for Jennifer Hillman Jackson
17 months ago by
Jennifer Hillman Jackson ♦ 25k
United States
Jennifer Hillman Jackson ♦ 25k wrote:

Hello,

GATK tools are deprecated in Galaxy. Alternative tools for variant analysis are in our tutorials here: https://galaxyproject.org/learn/

If you do run into a tool that does not have your genome natively indexed on the server you are working on, a Custom reference genome can be used. This is how: https://galaxyproject.org/learn/custom-genomes/

Thanks! Jen, Galaxy team
ADD COMMENT • link written 17 months ago by Jennifer Hillman Jackson ♦ 25k
Please log in to add an answer.

Similar posts • Search »

Content
Help
Access
Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 16.09
Traffic: 172 users visited in the last hour