Question: RNA STAR FATAL PARAMETER ERROR: limitGenomeGenerateRAM=31000000000is too small for your genome
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gravatar for Kaylinnette.Pinet
17 months ago by
Kaylinnette.Pinet • 0 wrote:

I am running RNA STAR to align RNA seq reads to the X. laevis transcriptome, which I uploaded from NCBI, but this is a very large genome of about 3.3x10^9. The current genome size limit for RNA STAR is for the human genome. Is there any workaround for this issue?

rna-seq alignment • 613 views
ADD COMMENT • link • modified 17 months ago by Jennifer Hillman Jackson ♦ 25k • written 17 months ago by Kaylinnette.Pinet • 0
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gravatar for Jennifer Hillman Jackson
17 months ago by
United States
Jennifer Hillman Jackson ♦ 25k wrote:

Hello,

The genome is too large to run with this tool at http://usegalaxy.org. Alternatives ways to use Galaxy are explained here: https://galaxyproject.org/choices/

We will be adding native indexes for this tool in the near future.

Thanks! Jen, Galaxy team

ADD COMMENT • link written 17 months ago by Jennifer Hillman Jackson ♦ 25k
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