Question: RNAseq analysis starter
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gravatar for bassam.abomoelak
8 months ago by
bassam.abomoelak10 wrote:

I just received my data for RNAseq and I appreciate guiding me through the steps of analysis. Thanks

rna-seq • 333 views
ADD COMMENTlink modified 8 months ago • written 8 months ago by bassam.abomoelak10
4
gravatar for Mo Heydarian
8 months ago by
Mo Heydarian790
United States
Mo Heydarian790 wrote:

Hello,

Have a look at the tutorials on transcriptomics from the Galaxy Training Network: http://galaxyproject.github.io/training-material/topics/transcriptomics/

There are two tutorials there, one for reference based RNA-seq analysis and another for de novo transcriptome reconstruction RNA-seq analysis. All of the tools in the latter can be used on useGalaxy.org .

These tutorials have step-by-step instructions for running the tools and screenshots to guide you along the way.

Best of luck with your analysis.

Cheers,

Mo Heydarian

ADD COMMENTlink written 8 months ago by Mo Heydarian790
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gravatar for bassam.abomoelak
8 months ago by
bassam.abomoelak10 wrote:

Dear Mo, Thanks so much for your reply. I will start soon navigating the system. Bassam

ADD COMMENTlink written 8 months ago by bassam.abomoelak10
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gravatar for bassam.abomoelak
8 months ago by
bassam.abomoelak10 wrote:

some of my fastq files are above 7GB. I know I can't upload from my computer. Is pasting the files link easier than FTP route?. if so, can I transfer them as folder or one by one?. Thanks for help

bassam

ADD COMMENTlink written 8 months ago by bassam.abomoelak10
0
gravatar for Mo Heydarian
8 months ago by
Mo Heydarian790
United States
Mo Heydarian790 wrote:

Hi Bassam,

If you have links to the files you can add them with the "Paste/Fetch data" option in the upload portal. If you would like help setting up a FTP connection, please see this guide: https://galaxyproject.org/ftp-upload/

Thanks for using Galaxy!

Cheers,

Mo Heydarian

ADD COMMENTlink written 8 months ago by Mo Heydarian790
0
gravatar for bassam.abomoelak
8 months ago by
bassam.abomoelak10 wrote:

My RNAseq files in txt.bZ2 format. Is there anyway to convert them into fastq.gz?. Thanks

Bassam

ADD COMMENTlink written 8 months ago by bassam.abomoelak10
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