Question: Tophat vs HISAT
1
gravatar for Beatriz Ramos
7 months ago by
Beatriz Ramos10 wrote:

Hi, I've been using Tophat to align RNA-seq reads followed by Cufflinks to estimate FPKM. Now that Tophat is out of the tools list, what is the main difference between using Tophat and HISAT?

rna-seq tophat cufflinks • 840 views
ADD COMMENTlink modified 7 months ago by Jennifer Hillman Jackson23k • written 7 months ago by Beatriz Ramos10
0
gravatar for Anton Nekrutenko
7 months ago by
Penn State
Anton Nekrutenko1.7k wrote:

HiSat (in particular HiSat2 that is currently on Galaxy) is the next generation of spliced aligner from the same group that have developed TopHat. It is MUCH faster and you can use its output for your old pipeline as well. This tutorial (https://galaxyproject.org/tutorials/rb_rnaseq/#read-mapping) explains how HiSat2 works.

ADD COMMENTlink modified 7 months ago • written 7 months ago by Anton Nekrutenko1.7k
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