Question: Metagenomics workflow / tutorial using paired-end sequencing (HiSeq)
0
gravatar for Darshan Baral
13 months ago by
Darshan Baral0 wrote:

I came across this galaxy workflow that was originally used with 454 sequences.

https://usegalaxy.org/u/aun1/p/windshield-splatter

I was wondering if it would also work with Hi-seq data (HiSeq 2500 2x125 PE)

ADD COMMENTlink modified 13 months ago by Jennifer Hillman Jackson25k • written 13 months ago by Darshan Baral0
0
gravatar for Jennifer Hillman Jackson
13 months ago by
United States
Jennifer Hillman Jackson25k wrote:

Hello,

The most current metagenomics tutorial with a Galaxy workflow is here: http://galaxyproject.github.io/training-material/Metagenomics/

The Windshield Splatter publication's workflow was designed for single end sequencing. It is also a bit outdated, so you will not find several of the tools at the Galaxy Main public server at http://usegalaxy.org anymore (if you plan to do the analysis there).

However, all of the original tools are in the Tool Shed. These are for use in a local or cloud Galaxy. Also, histories, datasets, and/or workflows can be exported/imported between Galaxy servers. The entire analysis is reproducible.

Thanks! Jen, Galaxy team

ADD COMMENTlink written 13 months ago by Jennifer Hillman Jackson25k
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