Question: Bam to BigWig conversion fail
0
gravatar for itiniakou
21 months ago by
itiniakou • 0
itiniakou • 0 wrote:

I tried converting my sorted bam files to bigwig (my build is mm10) and got this error: 1 is not found in chromosome sizes file

can anybody help me figure this out?

galaxy bam • 682 views
ADD COMMENT • link • modified 21 months ago by Devon Ryan • 1.9k • written 21 months ago by itiniakou • 0
1
gravatar for Devon Ryan
21 months ago by
Devon Ryan • 1.9k
Germany
Devon Ryan • 1.9k wrote:

Your BAM file was aligned against a genome using Ensembl chromosome names, but the Galaxy site you're using apparently uses UCSC chromosome names. If the instance you're using have deepTools installed, then you can use bamCoverage to create a bigWig file, since bamCoverage doesn't care whether you call the first chromosome 1 or chr1.

ADD COMMENT • link written 21 months ago by Devon Ryan • 1.9k
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