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Question: start with BAM file
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gravatar for dessaletesfahun
21 months ago by
dessaletesfahun • 0
dessaletesfahun • 0 wrote:

Hi, I have RNASeq data mapped outside. indeed I have both FASQ files and BAM files. is it possible to start with BAM files for differential gene expression in galaxy? how?

how to upload large file size? I am unable to upload a single file with file size greater that 2GB?
rna-seq galaxy bam • 717 views
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modified 21 months ago by jm.keith • 60 • written 21 months ago by dessaletesfahun • 0
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gravatar for jm.keith
21 months ago by
jm.keith • 60
jm.keith • 60 wrote:

You need to use an FTP to transfer large files to Galaxy. https://wiki.galaxyproject.org/FTPUpload
ADD COMMENT • link written 21 months ago by jm.keith • 60
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gravatar for dessaletesfahun
21 months ago by
dessaletesfahun • 0
dessaletesfahun • 0 wrote:

thanks, I tried it with but Filezilla couldn't connect to the galaxy FTP server. I tried with as follow. am I right? host = main.g2.bx.psu.edu
ADD COMMENT • link written 21 months ago by dessaletesfahun • 0
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gravatar for jm.keith
21 months ago by
jm.keith • 60
jm.keith • 60 wrote:

No, that's not correct. The first line of the page tells you:

"Please note the updated server to use for FTP: usegalaxy.org. This replaces the prior server "main.g2.bx.psu" in all materials below."

http://usegalaxy.org, for Galaxy Main

Also, I recommend checking out the video on that page. It's short but helpful.

Good luck
ADD COMMENT • link modified 21 months ago • written 21 months ago by jm.keith • 60
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gravatar for jm.keith
21 months ago by
jm.keith • 60
jm.keith • 60 wrote:

Also, I recommend searching the forum for your topic before posting. If it's already been asked/answered before, chances are likely that your posts will be ignored.

Example- https://biostar.usegalaxy.org/p/20545/
ADD COMMENT • link written 21 months ago by jm.keith • 60
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