Hi, I'm very new to Galaxy and RNA-seq in general. I can't seem to figure out why we are getting this problem every time I try to run HISAT on one of my fastq file.. I've been using Human genome hg38 as the reference genome but I've also tried the other 5 as well and I either get 0% alignment or this error message. What am I doing wrong?
(ERR): hisat2-align died with signal 11 (SEGV) (core dumped) [E::sam_parse1] SEQ and QUAL are of different length [W::sam_read1] parse error at line 483 [bam_sort_core] truncated file. Continue anyway.