I need to do TopHat my groomed data files, which are pair-ended. I groomed my files without any problem but I get error when I did TopHat. It is paired end (so downloaded File 1 and File 2 for each sample - I have two samples.). I choose hg38 as they were Human cells. I do not understand the exact reason of the error and would really apppreciate if you could help me about it. Thank you very much!