Question: Problems displaying data when using Drosophila dm6 reference genome
gravatar for nevil
18 months ago by
United States
nevil20 wrote:

I have been trying to analyze data using the most recent Drosophila genome version (dm6). Despite having no problem with older data sets using the previous version (dm3) I am not able to easily visualize the data, especially using UCSC.

Using the tool to convert both BedGraphs and Wig files to bigWig files (Wig/BedGraph-to-bigWig) results in the same error:

Couldn't open /galaxy-repl/localdata/chrom/dm6.len , No such file or directory Fatal error: Matched on Error Error running wigToBigWig.

Also these datasets fail to open when I attempt to visualize them with both IGV and IGB.

My only luck has come by keeping a small portion of the data and uploading it directly to the UCSC genome browser as a Wig file, since the files are too large to be uploaded completely.

Any advice or a way around this problem would be appreciated!

software error galaxy • 502 views
ADD COMMENTlink written 18 months ago by nevil20

This was a problem earlier that was corrected. Testing now to find out what the current issue is. More feedback soon. Jen, Galaxy team

ADD REPLYlink written 18 months ago by Jennifer Hillman Jackson25k
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