Question: Can't connect to the ftp server
gravatar for a.m.iyer
23 months ago by
a.m.iyer0 wrote:

Dear Galaxy,

I am trying to upload my RNA sequencing data onto the FTP server so that I can access them on Galaxy for analysis. I use Filezilla for the transfer. But every time I get the following error:

Status: Resolving address of Status: Connecting to Error: Connection timed out after 20 seconds of inactivity Error: Could not connect to server

Is there some problem with the server at the moment? I used the same user email id and password that I use to login on Galaxy, so I can't imagine its a problem with the login details.

Could you help me with this problem?

Thanking you in advance, Anand

upload ftp galaxy • 1.5k views
ADD COMMENTlink modified 23 months ago • written 23 months ago by a.m.iyer0

The connection works fine for me. Are you able to connect to a different FTP server? Were you able to connect to Galaxy FTP in the past? Also please try switching to 'passive mode' for the ftp connection.

ADD REPLYlink modified 23 months ago • written 23 months ago by Martin Čech ♦♦ 4.8k

Thanks for your suggestions. I think I found the problem. It probably has to do with the internet settings at the hospital where I work. I'll try later from my home connection. Thanks a lot for the quick response and suggestions!

ADD REPLYlink written 23 months ago by a.m.iyer0
gravatar for Jennifer Hillman Jackson
23 months ago by
United States
Jennifer Hillman Jackson25k wrote:


Are you using an FTP client? Filezilla is one choice.

How to FTP

  • First, double check that the server name is If you are working somewhere else, then use that server's URL. If a local, you must enable FTP first.
  • Second, double check your email/password. This is the same as you use to log into Galaxy. The account must be activated (confirmation email).
  • Full instructions here:

I also had no problems loading data (same as Martin). The server did take a while to connect, but after that transfer went fine, even for larger files.

Best, Jen, Galaxy team

ADD COMMENTlink written 23 months ago by Jennifer Hillman Jackson25k
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