Question: Motif Search from Bed file
0
gravatar for reubenmcgregor88
2.2 years ago by
reubenmcgregor8850 wrote:

Hi all,

I have recently generated a list of motifs of TF binding sites within peaks of my ChIPseq experiment. However this list tell me how many times the motif is present within my BED file, not where, i.e. which peaks have the motif associated with them.

I could take all of the sequences of interest in the BED file and run them through JASPAR for example but this seems like a very biased way of doing it.

Is it possible in Galaxy to search within a BEDfile for a specific motif which outputs in which BEDpeak the motif was found?

If not is there any other way of doing this?

Thanks in advance to anyone who can help me.

cistrome.org tool motif chip-seq • 1.4k views
ADD COMMENTlink modified 2.2 years ago by Jennifer Hillman Jackson25k • written 2.2 years ago by reubenmcgregor8850

Which tool did you use (name, version)?

What server was the query run against (by URL if public, or note if local or cloud).

ADD REPLYlink written 2.2 years ago by Jennifer Hillman Jackson25k

Hi Jennifer,

I used "SeqPos motif tool (version 1.0.0) " in the public "cistrone" at http://cistrome.org/ap/

Hope that makes sense?

Cheers

ADD REPLYlink modified 2.2 years ago • written 2.2 years ago by reubenmcgregor8850
1
gravatar for Jennifer Hillman Jackson
2.2 years ago by
United States
Jennifer Hillman Jackson25k wrote:

Hello,

I suspect that this is the expected output from that tool, but you could contact the owners of that instance for tool usage support. It is possible that they have recommendations to capture the information or may be willing to open an enhancement request.

They have a google user group - see this wiki: https://wiki.galaxyproject.org/PublicGalaxyServers#Cistrome_Analysis_Pipeline

Thanks, Jen, Galaxy team

ADD COMMENTlink written 2.2 years ago by Jennifer Hillman Jackson25k

Thanks as always Jen :)

ADD REPLYlink written 2.2 years ago by reubenmcgregor8850
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