Question: Cloudman instance: Tophat error
0
gravatar for karmella.haynes
18 months ago by
United States
karmella.haynes40 wrote:

Hello community,

I am encountering the following error when I try to run Tophat Galaxy Version 0.9 within my Cloudman instance:

[bam_header_read] EOF marker is absent. The input is probably truncated. [bam_header_read] invalid BAM binary header (this is not a BAM file). [bam_index_core] Invalid BAM header.[bam_index_build2] fail to index the BAM file. Fatal error: Tool execution

My inputs are fastq.gz files that I trimmed (outside of Galaxy) with Trimmomatic, then uploaded as fastqsanger (Human hg19).

Am I using the wrong type of input?

Thank you for your help.

ADD COMMENTlink modified 18 months ago by Jennifer Hillman Jackson24k • written 18 months ago by karmella.haynes40
0
gravatar for Jennifer Hillman Jackson
18 months ago by
United States
Jennifer Hillman Jackson24k wrote:

Hello,

The first pass solution is to allocate more memory for the analysis.

This is how to scale up the head/worker nodes on a cloudman instance (see item 5): https://wiki.galaxyproject.org/CloudMan

Best, Jen, Galaxy team

ADD COMMENTlink written 18 months ago by Jennifer Hillman Jackson24k
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