Question: Using The Public Server For Analysis
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gravatar for David Martin
7.6 years ago by
David Martin50
David Martin50 wrote:
Hello, I have a total 84 GB of Illumina reads (20 lanes total). I wasn't sure if I could analyze this amount of data on the public server or if this would bog down the system. I am looking to do a gene expression comparison between two groups of 10 animals using Tophat and Cufflinks. Can anyone tell me if this is okay or if I must try and use the Cloud for this analysis.. I am assuming the FTP option is the best for uploading this data. Thank you, David Martin To: Rory Kirchner Cc: Martin, David A.; galaxy-user@bx.psu.edu Subject: Re: [galaxy-user] Galaxy for gene expression comparison Hi all, I merged Rory's changes into galaxy-central, so Cuffdiff now supports replicates. I'll see what I can do for Cuffcompare; in the near-term, repeated merging using Cuffcompare will produce a GTF file that is both correct and usable with Cuffdiff. Thanks, J.
rna-seq cufflinks • 726 views
ADD COMMENTlink modified 7.5 years ago by Jennifer Hillman Jackson25k • written 7.6 years ago by David Martin50
0
gravatar for Jennifer Hillman Jackson
7.5 years ago by
United States
Jennifer Hillman Jackson25k wrote:
Hello David, Given the data size, using a local instance is your best option right now. Fairly soon, using the cloud would also work (as long as the analysis keeps total size under the 1TB). There are a few issues that prevent us from recommending the cloud right now, but we are actively working to bring functionality up to full speed, so feel free to check back soon (~ few weeks) if you'd like an update. Best wishes for your project, Jen Galaxy team -- Jennifer Jackson http://usegalaxy.org http://galaxyproject.org
ADD COMMENTlink written 7.5 years ago by Jennifer Hillman Jackson25k
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